Multilayered Polyelectrolyte Microcapsules: Interaction with the Enzyme Cytochrome C Oxidase
نویسندگان
چکیده
منابع مشابه
Multilayered Polyelectrolyte Microcapsules: Interaction with the Enzyme Cytochrome C Oxidase
Cell-sized polyelectrolyte capsules functionalized with a redox-driven proton pump protein were assembled for the first time. The interaction of polyelectrolyte microcapsules, fabricated by electrostatic layer-by-layer assembly, with cytochrome c oxidase molecules was investigated. We found that the cytochrome c oxidase retained its functionality, that the functionalized microcapsules interacti...
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Cytochrome c oxidase binds protonophore carbonyl cyanide 3-chlorophenylhydrazone (CCCP) with high affinity. There are 1.46 high-affinity binding sites per cytochrome c oxidase for CCCP with dissociation constant 2.7 x 10(-7) mol/l. The bond between the CCCP and cytochrome c oxidase accomplishes through the group on cytochrome c oxidase with pKa 6.64 and is based on the electrostatic interaction...
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The present study focuses on the formation of microcapsules containing catalytically active L-asparaginase (L-ASNase), a protein drug of high value in antileukemic therapy. We make use of the layer-by-layer (LbL) technique to coat protein-loaded calcium carbonate (CaCO3) particles with two or three poly dextran/poly-L-arginine-based bilayers. To achieve high loading efficiency, the CaCO3 templa...
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In the preceding paper the oxidation of substrates by “indophenol oxidase” was demonstrated to be a joint action of cytochrome and cytochrome oxidase. It was further shown that with a given amount of oxidase the velocity of hydroquinone oxidation reached a maximum as the amount of added cytochrome was increased. The latter fact immediately suggested the probability that the rapid aerobic oxidat...
متن کاملThe Reaction of Cytochrome Oxidase with Cytochrome c*
In recent work on the reaction between oxygen and reduced cytochrome oxidase made by the method of Yonetani (1)) Gibson and Greenwood (2) found that the reaction is complex and that at least two spectroscopically distinguishable reactions must take place. The kinetic changes were interpreted in terms of a scheme proposed by Keilin and Hartree (3), according to which cytochrome oxidase preparati...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2014
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0112192